FastQCFastQC Report
Mon 14 Jul 2014
S12_GGCTAC_L008_R1_001.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename S12_GGCTAC_L008_R1_001.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 32776281
Filtered Sequences 0
Sequence length 101
%GC 48

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[FAIL] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC 47327 0.14439405129581356 TruSeq Adapter, Index 11 (100% over 50bp)

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAGAA 8192495 2.5055523 5.5847106 2
CTCCT 6223875 2.0578396 5.4240894 1
CTTCA 6452060 2.0428054 5.000954 1
CTCCA 5956855 1.9708014 6.6691747 1
CTTGG 4993775 1.67587 5.1010623 1
CTGGA 4877380 1.6378464 6.1415763 1
Produced by FastQC (version 0.10.1)