Transcription Factor Family: VOZ
Mitsuda et al. 2004: A 38-bp pollen-specific cis-acting region of the AVP1 gene is involved in the expression of the Arabidopsis thaliana V-PPase during pollen development. Here, we report the isolation and structural characterization of AtVOZ1 and AtVOZ2, novel transcription factors that bind to the 38-bp cis-acting region of A. thaliana V-PPase gene, AVP1. AtVOZ1 and AtVOZ2 show 53% amino acid sequence similarity. Homologs of AtVOZ1 and AtVOZ2 are found in various vascular plants as well as a moss, Physcomitrella patens. Promoter-beta-glucuronidase reporter analysis shows that AtVOZ1 is specifically expressed in the phloem tissue and AtVOZ2 is strongly expressed in the root. In vivo transient effector-reporter analysis in A. thaliana suspension-cultured cells demonstrates that AtVOZ1 and AtVOZ2 function as transcriptional activators in the Arabidopsis cell. Two conserved regions termed Domain-A and Domain-B were identified from an alignment of AtVOZ proteins and their homologs of O. sativa and P. patens. AtVOZ2 binds as a dimer to the specific palindromic sequence, GCGTNx7ACGC, with Domain-B, which is comprised of a functional novel zinc coordinating motif and a conserved basic region. Domain-B is shown to function as both the DNA-binding and the dimerization domains of AtVOZ2. From highly the conservative nature among all identified VOZ proteins, we conclude that Domain-B is responsible for the DNA binding and dimerization of all VOZ-family proteins and designate it as the VOZ-domain.
There are 3 transcription factors which have been predicted for 3D structure in this family:
| Transcription Factor ID | Gene Description |
| AT1G28520.1 | Description: VOZ1 (VASCULAR PLANT ONE ZINC FINGER PROTEIN); transcription activator; Organism: Arabidopsis thaliana; Chromosome: 1; GeneType: Protein Coding; NCBI gene id: 839752 |
| AT1G28520.2 | Description: VOZ1 (VASCULAR PLANT ONE ZINC FINGER PROTEIN); transcription activator; Organism: Arabidopsis thaliana; Chromosome: 1; GeneType: Protein Coding; NCBI gene id: 839752 |
| AT2G42400.1 | Description: VOZ2 (VASCULAR PLANT ONE ZINC FINGER PROTEIN 2); transcription activator; Organism: Arabidopsis thaliana; Chromosome: 2; GeneType: Protein Coding; NCBI gene id: 818841 |